Toxicological assessment of nattokinase derived from Bacillus subtilis var. natto
نویسندگان
چکیده
منابع مشابه
In silico analysis of Nattokinase from Bacillus subtilis sp natto
Nattokinase or subtilisin NAT (EC 3.4.21.62) is one of the most remarkable enzymes produced by Bacillus subtilis sp. Natto, which posses direct fibrinolytic activity. The aim of this study is in silico analysis of Nattokinase structure and function. The three-dimensional structure of serine protease Nattokinase from Bacillus subtilis sp. natto was determined using homology modeling performed by...
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More than 20 unrelated proteins can form amyloid fibrils in vivo which are related to various diseases, such as Alzheimer's disease, prion disease, and systematic amyloidosis. Amyloid fibrils are an ordered protein aggregate with a lamellar cross-beta structure. Enhancing amyloid clearance is one of the targets of the therapy of these amyloid-related diseases. Although there is debate on whethe...
متن کاملComparative Fibrinolytic Activities of Nattokinases from Bacillus subtilis var. natto
Nattokinase is a potent fibrinolytic enzyme which was first found in a traditional Japanese soybean food. Two Nattokinase genes were cloned using chromosomal DNA from Bacillus subtilis (Ehrenberg 1835) Cohn 1872. Recombinant Natto-1.3 kb (GenBank accession number KF734090) and Natto-1.1 kb were expressed using a pQE-30 expression vector, and their fibrinolytic activities were studied. Natto-1.3...
متن کاملPurification and characterization of nattokinase from Bacillus subtilis natto B-12.
Bacillus subtilis natto B-12 was isolated from natto, a traditional fermented soybean food in Japan. A fibrinolytic enzyme (B-12 nattokinase) was purified from the supernatant of B. subtilis natto B-12 culture broth and showed strong fibrinolytic activity. The enzyme was homogenously purified to 56.1-fold, with a recovery of 43.2% of the initial activity. B-12 nattokinase was demonstrated to be...
متن کاملA newly derived protein from Bacillus subtilis natto with both antithrombotic and fibrinolytic effects.
Natto, steamed soybeans fermented by Bacillus subtilis natto, is a traditional Japanese food. We derived a purified protein layer, called NKCP as a trade mark, from B. subtilis natto fermentation. In the present study, we examined the fibrinolytic and antithrombotic effects of NKCP and identified its active component to clarify the fibrinolytic effect of NKCP observed in preliminary clinical tr...
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ژورنال
عنوان ژورنال: Food and Chemical Toxicology
سال: 2016
ISSN: 0278-6915
DOI: 10.1016/j.fct.2015.12.025